1H NMR spectroscopy reveals that mouse Hsp25 has a flexible C-terminal extension of 18 amino acids
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Quantification of organic and amino acids in beer by 1H NMR spectroscopy.
The quantification of organic and amino acids in beer using 1H NMR spectroscopy is demonstrated. Quantification was made both by integration of signals in the spectra together with use of calibration references and by use of partial least-squares (PLS) regression. Results from the NMR quantifications were compared with those obtained from determinations by amino acid analysis on HPLC and organi...
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During the course of an investigation into the biological activities and chemical properties of saliva parotin A it became necessary to study the chemical structure of this protein. Previous workers have reported that the molecular weight of saliva parotin A prepared from human saliva is 15,900 (Ito, Y. 1960). Proline, threonine, aspartic acid, and gluta mic acid are contained in saliva parotin...
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We describe a cost efficient C NMR spectroscopy experiment using a low-field NMR instrument and non-deuterated aqueous solutions that can be easily performed at teaching schools where high field instruments are not affordable. The experiment uses the effect of pH on the structure of amino acids, which affects the chemical shifts. By monitoring the changes in the C chemical shifts of the amino a...
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In our previous study, we compared the two α-amylase enzymes from Bacillus sp.KR8104, BKA∆(N44) and BKA∆(N44C193) which is the secreted form of it. The results indicated that the presence of 193 amino acids propeptide in the C-terminal of BKA∆(N44) changed its enzymatic parameters like an uncompetitive inhibitor in comparison to BKA∆(N44C193). In the present study, we cloned the DNA sequence of...
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Rhodopsin, the photosensitive pigment of rod vision, is a chromoprotein composed in equimolar parts of the protein opsin and the carotenoid retinene (1). Its absorption spectrum and molar extinction are known (2) and the kinetics of bleaching by light and of regeneration have been investigated (3). An upper limit of 40,000 has been set for its molecular weight (cattle) (1). Since rhodopsin is i...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1995
ISSN: 0014-5793
DOI: 10.1016/0014-5793(95)00770-a